Table 1.
Gene | Primer sequence | Product (bp) |
---|---|---|
TIF-IA | 5'- ttcaggatccaaatggcggcaccgctgcttcacacg -3' (forward) 5'- catgctcgagtcagaggggactgggttgcatgtacaac -3' (reverse) |
1970 |
EGFP-PAF53 | 5'- agatctcgagatggcggcggaggtgttgccgagtgcg -3' (forward) 5'- gcagaattcgctaggtaatcttcctccgctttgccaggc-3' (reverse) |
1260 |
18S rRNA to ITS (between 18S rRNA and 5.8S) |
5'- ttggatggtttagtgaggc-3' (forward) 5'- cacgcccttctttctctctc-3' (reverse) |
394 |
GAPDH | 5'- ggtgaaggtcggagtcaacg -3' (forward) 5'- caaagttgtcatggatgacc -3' (reverse) |
500 |