. 2015 Aug 7;4:e07467. doi: 10.7554/eLife.07467

Table 1.

Lamprey plasma binding is sensitive to drifted viruses by ELISA and HI

DOI: http://dx.doi.org/10.7554/eLife.07467.010

Table 1—source data 1.

Other animals show similar binding to sequential virus series. ELISA binding curves for lamprey plasma from Table 1 are plotted alongside PR8 immunized guinea pig, chicken and mouse sera against the same plated Sequential virus series. Each graph shows representative data on a single animal's sera. Percent change in area under curve between wt PR8 and Sequential 12 is shown on each graph (ΔAUC).

DOI: http://dx.doi.org/10.7554/eLife.07467.011

elife07467s001.pdf^{(1.3MB, pdf)}

DOI: 10.7554/eLife.07467.011

Table 1—source data 2.

More H1N1 isolates. ELISA binding curves for lamprey plasma from Table 1 against plated H1N1 isolates are plotted along with two additional isolates omitted from the Table.

DOI: http://dx.doi.org/10.7554/eLife.07467.012

elife07467s002.pdf^{(450.7KB, pdf)}

DOI: 10.7554/eLife.07467.012

Table 1—source data 3.

Anti-HA stem Ab binding curves used to normalize amounts of plated HA in Table 1. (A) ELISA binding curves for serially diluted anti-HA stem Ab (3A01) added to the Sequential virus series are show from each of the four experiments. (B) Same as (A) for the H1N1 isolate panel.

DOI: http://dx.doi.org/10.7554/eLife.07467.013

elife07467s003.pdf^{(603.7KB, pdf)}

DOI: 10.7554/eLife.07467.013

Virus	ELISA % ΔAUC^*	HI Titer^†
PR8	–	40
# Substitutions in HA head
3	15	10
6	−25	20
9	−46	<10
12	−59	<10
H1N1 isolates
A/Weiss/43	−61	–
A/Cameron/46	−50	–
A/Malaysia/54	−77	–

Equal amounts of each virus were plated and probed with lamprey plasma (L25) or mouse sera. Data from four independent experiments were normalized to the B_Max of the stem binding mAb 3A01 to allow precise comparison between viruses and replicates (Table 1—source data 3). Data were fit to a hyperbola and the percent change in area under curve (AUC) between PR8 and the indicated virus is reported. All curve-means were significantly different from PR8 by Two Way ANOVA followed by Tukey Multiple comparisons test, p < 0.001, except PR8 vs 3.

^†

Agglutination inhibition of four HAU of input virus by lamprey plasma (L27) occurred at the dilution reported.

HA, hemagglutinin.