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. Author manuscript; available in PMC: 2015 Oct 20.
Published in final edited form as: Angew Chem Int Ed Engl. 2014 Sep 4;53(43):11483–11487. doi: 10.1002/anie.201405362

Figure 4.

Figure 4

Comparison of the pre-tRNATyr processing activity at 55°C of the 4-RPP Pfu RNase P complex reconstituted in vitro under conditions employed for native MS (MS) and those previously optimized for function in vitro (PRA). [10a, 10d] NC, a negative control with pre-tRNATyr alone; PC, a positive control with in vitro reconstituted Escherichia coli RNase P and pre-tRNATyr; – and + are parallel reactions performed without and with Pfu RNase P, respectively. See supplement for additional details.