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. 2015 Jul 24;1(6):e1500154. doi: 10.1126/sciadv.1500154

Fig. 1. Effects of different lysine codons on mCherry reporter expression and mRNA stability.

Fig. 1

(A) Cartoon of reporter constructs used in electroporation experiments. (B) Western blot analyses of HA-X-mCherry constructs 48 hours after electroporation (HA and β-actin antibodies). (C) Normalized protein expression using LI-COR Western blot analyses or in vivo mCherry fluorescence measurement. β-Actin or fluorescence of coexpressed GFP construct was used for normalization of the data. Each bar represents the percentage of wild-type mCherry (WT) expression/fluorescence. (D) Normalized RNA levels of HA-X-mCherry constructs. Neomycin resistance gene was used for normalization of qRT-PCR data. Each bar represents the percentage of wild-type mCherry (WT) mRNA levels.