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. 2015 Aug 27;10(8):e0136732. doi: 10.1371/journal.pone.0136732

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© 2015 Urbanek et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) The effect of Ysc84 overexpression on endocytosis was assessed by measuring lifetime of different endocytic reporters. Movies of cells expressing fluorescently tagged different endocytic markers transformed with YSC84 and an empty plasmid were recorded. At least 30 patches were assessed for each transformant. Error bars are SD. The differences in lifetimes of endocytic markers were calculated using two-tailed Student’s t-test. *** indicates a P value of 0.002 and **** ≥ 0.001. (B) Kymographs were generated from individual patches of fluorescently tagged endocytic markers expressing an empty plasmid or overexpressing YSC84 using the multiple kymographs ImageJ plugin. Arrows mark retractions. (C) Spot tracking of Sla1-GFP patches was performed in cells expressing an empty plasmid or overexpressing YSC84 in manual tracking–ImageJ. Red spot–start, green spot–end. The time between spots is 1 second.