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. 2015 Aug 27;10(8):e0136732. doi: 10.1371/journal.pone.0136732

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© 2015 Urbanek et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — (A) The effect of Ysc84 mutants on Sla1-GFP life-time was assessed by measuring patch lifetime from movies recorded of cells expressing Sla1-GFP in ysc84 deletion strains transformed with YSC84 and its mutants. At least 30 patches were assessed for each transformant. Error bars are SD (B) Kymographs were generated from individual Sla1-GFP endocytic patches in wild-type or Ysc84 mutant cells by using multiple kymographs ImageJ plugin. Spot tracking was created by following a movement of individual Sla1-GFP patch in ImageJ (manual tracking); Pink arrows–indicate small retractions of KK mutant; Blue arrows–retractions of RL mutant (C) Patch intensity profile. ImageJ manual tracking plugin was used to generate single patch profile intensity. An average of 4 patches were tracked for Sla1-GFP, ysc84Δ cells carrying empty and YSC84. The intensity was normalized to the same maximum.