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Schematic of treatment regimen for WT and Nmp4−/− mice; group 1 mice were subjected to ovx or sham operation at 12 weeks of age and evaluated for bone loss 4-week postop (16 wk of age). Group 2 mice were ovx at 12 weeks of age and began PTH or vehicle therapy at 16 weeks of age for a duration of 4 and 8 weeks. Endpoint analyses included μCT, serum analysis for P1NP and CTXs, and dynamic histomorphometry.
