Figure 10.
Comparison of mRNA expression profiles derived from nondifferentiating (d 3) and osteogenic-differentiating (d 5–16) WT and Nmp4−/− cells. All transcript levels are compared with WT day 3 providing a time course of expression. mRNA profiles (A) Cxcl12, (B) Plaur, (C) Spp1, (D) Thbs2, (E) Col1a1 were derived from the TLDA system (Format 96a; Applied Biosystems) performed on a QuantStudio 7 Flex Real-Time PCR System and normalized with GusB. F, Sp7 mRNA profile qRT-PCRs were performed on an Eppendorf Mastercycler RealPlex2 using Rplp2 (Mm03059047_gH) as the normalizer, as previously described (Robling et al [13]). The day-16 WT sample is the average of 2 replicates. Comparison of profiles using GusB and Rplp2 as the normalizer showed no differences in the shape of the expression profiles.