Figure 4.

Ovx does not abrogate the expanded population of osteoprogenitors and CD8⁺ T cells in Nmp4^−/− mice. FACS analysis of BM and PBL osteoprogenitors, CD8⁺ T cells, and CD4⁺ T cells. A and D, The frequency of femoral BM and PBL CD45⁻/CD105⁺/CD146⁺/CD105⁺/nestin⁺ osteoprogenitor cells in WT and Nmp4^−/− mice at the end of 4 and 8 weeks of treatment with intermittent PTH or vehicle control. B and E, The frequency of BM and PBL CD8⁺ T cells from the WT and Nmp4^−/− mice. C and F, The frequency of BM and PBL CD4⁺ T cells from the WT and null mice. Data are average ± SD, number of mice/experimental group = 8–9. Statistical differences were determined using a two-way ANOVA, and significance was set at P ≤ .05.