Table 1.
Bone Loss Data
| WT |
Nmp4−/− |
Two-Way ANOVA P Values |
|||||
|---|---|---|---|---|---|---|---|
| Sham | ovx | Sham | ovx | Genotype | Treatment | Gene × Treat | |
| %Δ body weight | 2.48 ± 7.73 | 8.65 ± 5.48 | 4.14 ± 4.70 | 5.66 ± 2.94 | .69 | 0.03 | .17 |
| Uterine weight (g) | 0.10 ± 0.05 | 0.04 ± 0.02 | 0.10 ± 0.02 | 0.05 ± 0.02 | .30 | <0.0001 | .34 |
| Distal femur | |||||||
| BV/TV | 0.019 ± 0.004 | 0.012 ± 0.004 | 0.038 ± 0.011 | 0.021 ± 0.010 | <.0001 | <0.0001 | .06 |
| SMI | 3.818 ± 0.250 | 4.055 ± 0.357 | 3.387 ± 0.263 | 3.810 ± 0.294 | .0008 | 0.0011 | .32 |
| Tb.N (mm−1) | 2.554 ± 0.239 | 2.165 ± 0.385 | 3.128 ± 0.218 | 2.797 ± 0.276 | <.0001 | 0.0004 | .76 |
| Tb.Th (mm) | 0.040 ± 0.005 | 0.041 ± 0.005 | 0.039 ± 0.003 | 0.037 ± 0.004 | .08 | 0.87 | .23 |
| Tb.Sp (mm) | 0.393 ± 0.036 | 0.477 ± 0.097 | 0.317 ± 0.026 | 0.359 ± 0.037 | <.0001 | 0.0012 | .25 |
| L5 vertebra | |||||||
| BV/TV | 0.189 ± 0.028 | 0.177 ± 0.013 | 0.253 ± 0.019 | 0.212 ± 0.019 | <.0001 | 0.0004 | .05 |
| Tb.N (mm−1) | 3.797 ± 0.513 | 3.580 ± 0.285 | 4.491 ± 0.345 | 4.022 ± 0.254 | <.0001 | 0.0091 | .32 |
| Tb.Th (mm) | 0.050 ± 0.003 | 049 ± 0.002 | 0.056 ± 0.002 | 0.053 ± 0.002 | <.0001 | 0.0032 | .02 |
| Tb.Sp (mm) | 0.227 ± 0.023 | 0.229 ± 0.013 | 0.202 ± 0.020 | 0.214 ± 0.012 | .0013 | 0.25 | .44 |
| Serum P1NP | WT: Nmp4−/− |
P Values [G; T; G × T]4 wks | P Values [G; T; G × T]12 wks | ||
|---|---|---|---|---|---|
| Preop | Postop4 wks | Postop12 wks | |||
| 5.62 ± 1.21: 6.02 ± 1.41 | 5.83 ± 1.41: 4.77 ± 1.22 | 3.15 ± 0.65: 2.81 ± 0.76 | .34; .16; .05 | .95; <.0001; .36 | |
| Serum CTX | WT: Nmp4−/− |
P Values [G; T; G × T]4 wks | P Values [G; T; G × T]12 wks | ||
|---|---|---|---|---|---|
| Preop | Postop4 wks | Postop12 wks | |||
| 13.66 ± 2.43: 13.37 ± 1.88 | 12.96 ± 3.04: 13.53 ± 2.58 | 11.47 ± 2.24: 9.36 ± 1.22 | .92; .46; .96 | .14; .0003; .18 | |
The % change body weight, uterine weight, and μCT of distal femur and L5 vertebra from WT and Nmp4−/− mice after ovx or sham operation 4-week postop. Serum bone formation (P1NP) and bone resorption (CTX) marker levels were compared in mice previous to the operation (preop) and 4 and 12 weeks after surgery (postop). The 12-week postop data were obtained from the vehicle-control treatment groups. Data are average ± SD, number of mice/experimental group = 8–14 (4 mice in WT sham uterine weight). Statistical significance was set at P ≤ .05, and differences were determined using a two-way ANOVA.