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Summary of Kinetic Data for P450 21A2 Mutant Proteins With the PROG or 17OHP Substrates
| P450 21A2 | Substrate | kcat (min−1) | Km (μM) | kcat/Km (μM−1 min−1) | Activity (%), wt |
|---|---|---|---|---|---|
| wt | PROG | 170 ± 4 | 0.21 ± 0.03 | 800 ± 120 | (100) |
| 17OHP | 240 ± 5 | 1.5 ± 0.1 | 160 ± 11 | (100) | |
| G65E (SW) | PROG | 3.7 ± 0.2 | 5.5 ± 0.8 | 0.67 ± 0.10 | 0.08 |
| 17OHP | 1.9 ± 0.2 | 13 ± 2 | 0.14 ± 0.03 | 0.08 | |
| T296N (SV/SW) | PROG | 0.46 ± 0.16 | 1.1 ± 0.2 | 0.42 ± 0.16 | 0.05 |
| 17OHP | 2.7 ± 0.3 | 0.46 ± 0.2 | 6.0 ± 2.7 | 3.7 |
PROG, progesterone; 17OHP, 17α-OH progesterone.
