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. 2015 Aug 27;10(8):e0136829. doi: 10.1371/journal.pone.0136829

Fig 1. Confirmation of EGFR expression as a target for PIT in MDAMB231 and MDAMB468 cells, and evaluation of in vitro PIT.

Fig 1

(A) Expression of EGFR in MDAMB231 cells was examined with FACS. (B) Expression of EGFR in MDAMB468 cells was examined with FACS. (C) MDAMB231 and MDAMB468 cells were incubated with cet-IR700 for 6 h and observed by microscopy. Fluorescence intensities of MDAMB468 were higher than MDAMB231. Necrotic cell death was observed upon excitation with 2 J/cm2 of NIR light (after 15min). Bar = 20 μm. (D) Membrane damage of MDAMB231 cells induced by PIT was measured with PI staining (dead cell count), which increased in a light dose-dependent manner (n = 5, *p < 0.001, vs. untreated control, by Student’s t test). (E) PI staining showed membrane damage of MDAMB468 cells (n = 5, *p < 0.001, vs. untreated control, by Student’s t test).