Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 26;16(8):939–954. doi: 10.15252/embr.201540352

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Parkin His302 is required for optimal activation of Parkin ubiquitin E3 ligase activity at mitochondria in response to PINK1 activation by CCCP — Wild-type HeLa cells were transfected with untagged wild-type (WT), and Ser65Ala (S65A)-, His302Ala (H302A)- or Cys431Phe (C431F)-mutant Parkin and stimulated with 10 μM of CCCP or DMSO for 6 h. Mitochondrial enriched extracts were incubated with a ubiquitin-binding resin derived from His-Halo-Ubiquilin UBA-domain tetramer (UBA^UBQLN¹). Captured ubiquitylated proteins were subjected to immunoblotting with CISD1 and ubiquitin antibodies. In parallel, mitochondrial input extracts were immunoblotted with Tomm70 antibody.