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. 2015 Jun 29;16(8):965–974. doi: 10.15252/embr.201540084

Figure 4.

Figure 4

Pelo possibly functions at the level of translation in transposon silencing

  • A Pelo and Hbs1 interaction was detected by the yeast two-hybrid assay.
  • B Co-immunoprecipitation of Pelo with Hbs1 in ovarian extracts.
  • C–G Quantitative RT–PCR to detect the relative amount of transposon mRNAs from ovaries of the indicated genotypes. Fold changes were compared to heterozygous controls. (C) Transposon mRNAs were mildly increased in Hbs1−/− ovaries at 29°C. (D) Hbs1 mutation further enhanced HeT-A up-regulation in pelo mutant ovaries. (E) Transgene expression of Pelo (P210A) could not prevent HeT-A and ZAM up-regulation in pelo mutant ovaries. (F, G) Transgene expression of RpS30a reduced TE levels in pelo1/PB60 but not AubQC42/HN2 (G) ovaries. Values are means ± SEM, n = 3–5. n.s., not significant; *P < 0.05, **P < 0.01, t-test.
  • H, I Germline expression of RpS30a partially restored egg production (H) and hatching rate (I) of pelo1/PB60 females, similar to the effect caused by germline expression of pelo. Values are means ± SEM, n = 16–40 females.