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. 2015 Jul 3;16(8):986–994. doi: 10.15252/embr.201540330

Figure 3.

Figure 3

INO80 and ANP32E work together to promote RAD51 foci formation following irradiation, which is rescued by the depletion of H2A.Z

  1. IR induced RPA focus formation in A549 cells treated with siControl, siANP32E, siANP32E/siINO80 or siANP32E/siH2A.Z. Left hand panel: representative images. Right hand panel: mean number of foci ± SD.
  2. IR induced RAD51 focus formation in A549 cells treated with siControl, siANP32E, siANP32E/siINO80 or siANP32E/siH2A.Z. Left hand panel: representative images. Right hand panel: mean number of foci ± SD.
  3. Western blot analysis showing efficiency of siRNA depletion. Ku80 is used as a loading control.

Data information: Scale bars represent 5 μm. All data (A, B) are from a minimum of 3 independent assays. NS, not significant; *P ≤ 0.05; ***P ≤ 0.001 by Student’s t-test.