Figure 4.

Replication of movement-deficient mutants

Nicotiana benthamiana plants were agroinfiltrated with full-length WT and mutant viral clones. A replication-deficient mutant, ΔGDD, was used as a negative control. At 6 d.p.i., total RNA and protein were extracted from infiltrated patches. Virus replication was detected with strand-specific RT–PCR. Ethidium bromide staining of 28S rRNA was used as a control for RNA quality. Protein extracts were analysed by Western blot for the presence of CP and P3 (V5 epitope). Ponceau S staining of RuBisCO large subunit (RuBP-L) was used as a loading control.