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. 2015 Jul 1;16(8):1037–1050. doi: 10.15252/embr.201540159

Figure 6.

Figure 6

Impaired muscle regeneration in Brm−/− mice

  • A Schematic representation of the experimental setting, showing the time of notexin-mediated muscle injury and tissue analysis (n = 4).
  • B Regeneration of tibialis anterior (TA) muscles from wild-type (WT) and Brm null (Brm−/−) 2.5-month-old mice was evaluated by morphological criteria (hematoxylin and eosin (H&E) staining), the presence of regenerating myofibers (laminin/embryonic MyHC) and the presence of inflammatory infiltration (laminin/CD45) 7 days after notexin injury. Scale bar, 50 μm.
  • C, D Analysis of cross-sectional area (CSA) of muscles represented as mean of CSA in WT and Brm−/− mice uninjured or post-injury.
  • E Quantification of % of area occupied by CD45-positive cells in randomly selected fields.
  • F Quantification of MyHC-positive fibers in randomly selected fields.
  • G, H In addition to notexin injury, as indicated in (A), WT and Brm−/− mice (2.5 months old) received intraperitoneal injection of EdU. Immunohistochemistry for Pax7, laminin and EdU were performed in sections from TA muscles to detect proliferating satellite cells (Pax7/EdU double-positive cells within laminin-positive fibers), and its relative quantification. Scale bar, 50 μm.

Data information: Data are presented as average ± SEM (n > 3). P-value was calculated using unpaired Student’s t-test *P < 0.05; **P < 0.01; ***P < 0.001.