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Efficient Cyclin D1 downregulation upon siRNA transfection
Immunofluorescence analysis of cyclin D1 expression in C2C12 cells depleted for Brm (siBrm) or Brg1 (siBrg1) in GM or DM 48 h upon delivery of siRNA against cyclin D1 or control sequence. Note that cyclin D1 is effectively depleted (bottom panel) and is specifically upregulated in DM condition only in Brm-depleted cells (upper panel). Scale bar, 50 μm.
