Ectopic expression of BRM rescues the myogenic differentiation defects in Brm^−/− cells

1. Luciferase assay in WT or Brm^−/− mouse embryonic fibroblasts (MEFs) that were transfected with 4RELuc reporter and infected with MyoD. MEFs were transfected with the indicated constructs in GM. 24 h after transfection, the cells were shifted in differentiation medium, and harvested 48 h later for luciferase assay. Luciferase values are expressed as fold induction relative to the activity measured in the absence of MyoD. Data are shown as average ± SEM (n = 3).
2. Western blot revealing the expression levels of the indicated proteins in WT or Brm^−/− MEFs under the conditions used for luciferase assay in (A).
3. Western blot of the indicated proteins after reintroduction of FLAG-tagged Brm in Brm^−/− MEFs. After 2 days in growing medium, cells were transferred to differentiation medium and harvested 48 h later.
4. Brightfield images of satellite cells isolated from muscles of WT and Brm^−/− mice, after reintroduction of Brm by retroviral expression vector. Scale bar, 50 μm.
5. Relative expression analysis in WT and Brm^−/− MEFs infected with MyoD and induced to differentiate by switch with DM medium. Samples were collected at the time points indicated from the onset of differentiation induction (DM0). The expression levels are shown as relative to DM0, and the graph is representative of three independent experiments.