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. 2015 Jun 26;146(1):59–69. doi: 10.1111/imm.12480

Figure 3.

Figure 3

Proliferative status of small intestine intraepithelial lymphocytes (SI-IELs) in polymeric immunoglobulin receptor-positive (pIgR+/+) mice and pIgR−/− mice. Mice were given distilled water containing 5-bromo-2′-deoxyuridine (BrdU; 0·8 mg/ml) for 14 days (pulse), after which they received distilled water without BrdU for an additional 10 days (pulse → chase). Bone marrow cells, thymocytes and SI-IELs were prepared from pIgR+/+ mice (■) or pIgR−/− mice (□) just after the ending of pulse and 10 days after the starting of chase. Cells were stained with FITC-conjugated anti-BrdU monoclonal antibody (mAb), or in the case of SI-IELs they were stained with phycoerythrin-conjugated mAbs specific for CD4, CD8α and CD8β, after which they were incubated with FITC-conjugated anti-BrdU mAb. Data are shown as mean ± SD of two to five mice. **P < 0·05, *P < 0·01.