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. 2015 Jun 26;146(1):59–69. doi: 10.1111/imm.12480

Figure 5.

Figure 5

Cytokine production by small intestine intraepithelial lymphocytes (SI-IELs) of polymeric immunoglobulin receptor-positive (pIgR+/+) mice and pIgR−/− mice. (a) Purified αβ-IELs and γδ-IELs from pIgR+/+ mice (■) and pIgR−/− mice (□) were stimulated with immobilized anti-CD3 monoclonal antibody (mAb) for 48 hr, after which the concentrations of interferon-γ (IFN-γ) and interleukin-17 (IL-17) in the culture supernatants were measured using ELISA. Results were expressed as mean ± SD of data from three independent experiments. (b) Total RNA was extracted from purified αβ-IELs and γδ-IELs from pIgR+/+ mice (■) and pIgR−/− mice (□), and mRNA levels of IFN-γ and IL-17 were examined by quantitative PCR. Relative density to GAPDH mRNA was expressed. (c) Intracellular IFN-γ was detected in CD4+ and CD8β+ subsets in SI-IELs isolated from pIgR+/+ mice and pIgR−/− mice.