Figure 4.

Interleukin-33 (IL-33) modulates T-cell immunity by inhibiting T helper type 1 (Th1)/Th17 differentiation and enhancing IL-10 production. Skin-transplanted animals were killed at Day 10 post-surgery, and draining lymph nodes (dLNs) were removed for analysis. Cell suspensions were prepared and cultured for 72 hr under polyclonal stimulation. After this time, supernatants were collected for cytokine quantification. (a) Graphs showing the amounts for interferon-γ (IFN-γ) (top) and IL-17 (bottom). (b) Graph depicting the amount of IL-10 obtained by ELISA. Bars correspond to the standard deviation (SD), and the statistical significance was assessed by analysis of Mann–Whitney test, *P = 0·05, **P = 0·01; two independent experiments, with two to four mice per group in each experiment.