Figure 2.

Adenanthin (ADNT) affects the redox balance in natural killer (NK) cells. (a) Relative reactive oxygen species (ROS) levels in NK cells incubated with hydrogen peroxide or ADNT measured by CM-H2-DCFDA fluorescence. (b) Dose- and time-dependent effects of ADNT on peroxiredoxin 1 (PRDX1) dimer/monomer presence in human NK cells, as assessed by electrophoresis in denaturing, non-reducing conditions, followed by immunoblotting. (c) Effects of N-ethylmaleimide (NEM) or ADNT on levels of glutathione (left-hand panel), glutathione disulphide (GSSG) (right-hand panel) in human NK cells. Data presented as the averages from two independent donors ± SEM. **P < 0·01.