Table 1.

Time course of serum metabolites during exercise.

Metabolite	Time (min)
	0			30			60
	WT	ACC2-KI	ACCD-KI	WT	ACC2-KI	ACCD-KI	WT	ACC2-KI	ACCD-KI
Glucose (mM)	8.40 ± 0.21	7.59 ± 0.22*	8.22 ± 0.36	11.85 ± 0.69#	10.04 ± 0.32#	11.90 ± 0.33#	11.30 ± 0.77#	10.06 ± 0.48#	11.17 ± 0.44#
NEFA (mEq/L)	0.97 ± 0.08	1.16 ± 0.07	1.13 ± 0.07	1.35 ± 0.09	1.20 ± 0.08	1.29 ± 0.08	1.55 ± 0.07	1.28 ± 0.06*	1.70 ± 0.13
Lactate (mM)	5.54 ± 0.32	5.49 ± 0.23	6.67 ± 0.37*	5.33 ± 0.31	5.26 ± 0.39	5.27 ± 0.34#	5.56 ± 0.60	5.19 ± 0.41	5.58 ± 0.18#
Glycogen (μmol/g w/w)	11.33 ± 0.37	8.96 ± 0.25*	7.88 ± 0.69*	ND	ND	ND	4.68 ± 0.25	4.80 ± 0.20	4.43 ± 0.47

ND, Not determined.

Male ACC2-KI, ACCD-KI and WT mice were run on a treadmill at 65% maximal running speed and blood was collected at basal 0 min and 30 and 60 min for determination of serum glucose, non-esterified fatty acids (NEFA) and lactate as described under methods. Glycogen levels were measured in muscle at rest (as previously reported (12)) and following 60 min treadmill running. Data expressed as means ± SEM. n = 6–7 mice.

^#P < 0.05 compared to basal (time = 0 min), same genotype.

^*P < 0.05 compared to WT, same condition.