Table 1.
Forward and reverse primer sequences for quantitative real-time PCR
| Gene | Primer sequence (5 3′) | |
|---|---|---|
| Forward | Reverse | |
| 18S | GCCGCTAGAGGTGAAATTCTTG | CATTCTTGGCAAATGCTTTCG |
| VCAM-1 | GAAGGAAACTGGAGAAGACAATCC | TGTACAAGTGGTCCACTTATTTCAATT |
| ICAM-1 | CACAAGGGCTGTCACTGTTCA | CCCTAGTCGGAAGATCGAAAGTC |
| MCP-1 | CTGTCTCAGCCAGATGCAGTTAA | AGCCGACTCATTGGGATCAT |
| IL-6 | AGAGACTTCCAGCCAGTTGC | AGCCTCCGACTTGTGAAGTG |
| IL-10 | CTGGCTCAGCACTGCTATGT | GCAGTTATTGTCACCCCGGA |
| TNF-α | AACACACGAGACGCTGAAGT | TCCAGTGAGTTCCGAAAGCC |
| ET-1 | TTGCTCCTGCTCCTCCTTGAT | TAGACCTAGAAGGGCTTCCTAGT |
| ENOS | AGGCATCACCAGGAAGAAGA | GGCCAGTCTCAGAGCCATAC |
| CD8 | CACTAGGCTCCAGGTTTCCG | CGCAGCACTTCGCATGTTAG |
| CD3e | AGTAATGAGCCAGCCGTGTC | ATGCTCCAGAAAGCGTTCCA |
| CD11c | CTGTCATCAGCAGCCACGA | ACTGTCCACACCGTTTCTCC |
| CD4 | ACCCTAAGGTCTCTGACCCC | TAGGCTGTGCGTGGAGAAAG |
| F4/80 | GCCATAGCCACCTTCCTGTT | ATAGCGCAAGCTGTCTGGTT |
| PAI-1 | AGCTGGGCATGACTGACATCT | GCTGCTCTTGGTCGGAAAGA |
| FoxP3 | CTCCAGTACAGCCGGACAC | GGTTGGGCATCAGGTTCTTG |