. 2015 Aug 28;10(8):e0136820. doi: 10.1371/journal.pone.0136820

Table 1. Description of the reference and target genes used for qPCR analysis.

Gene name	Gene Symbol	Accession number ^a	Primer sequence (5’-3’)	Amplicon size (pb)	Amplification efficiency ± SD ^b	Reference
Actin	ACT	BT013524	CGGTGACCACTTTCCGATCT	62	1.038 ± 0.0077	[14]
Actin	ACT	BT013524	TCCTCACCGTCAGCCATTTT	62	1.038 ± 0.0077	[14]
β-6 Tubulin	TUB	BT013153	TTGGTTTTGCACCACTGACTTC	84	0.987 ± 0.0070	[14]
β-6 Tubulin	TUB	BT013153	AAGCTCTGGCACTGTCAAAGC	84	0.987 ± 0.0070	[14]
Adenine phosphoribosiltransferase 1	APT	BT012816	GAACAGACAAGATTGAGATGCATGTA	60	0.999 ± 0.0072	[14]
Adenine phosphoribosiltransferase 1	APT	BT012816	CCACGAGGGCACGTTCA	60	0.999 ± 0.0072	[14]
Phytoene Desaturase	PDS	NM_001247166	GCCGATTGTGGAACATATTGAGTC	91	1.027 ± 0.0085	Our library
Phytoene Desaturase	PDS	NM_001247166	GACACTTCCATCCTCATTCAGCTC	91	1.027 ± 0.0085	Our library
Expressed protein	EXP	SGN-U346908	GCTAAGAACGCTGGACCTAATG	183	1.052 ± 0.0110	[11]
Expressed protein	EXP	SGN-U346908	TGGGTGTGCCTTTCTGAATG	183	1.052 ± 0.0110	[11]
TIP 41 (TAP42-interacting protein)	TIP41	SGN-U584254	ATGGAGTTTTTGAGTCTTCTGC	235	1.018 ± 0.0067	[11]
TIP 41 (TAP42-interacting protein)	TIP41	SGN-U584254	GCTGCGTTTCTGGCTTAGG	235	1.018 ± 0.0067	[11]
Clathrin adaptor complexes medium subunit	CAC	SGN-U314153	CCTCCGTTGTGATGTAACTGG	173	1.107 ± 0.0184	[11]
Clathrin adaptor complexes medium subunit	CAC	SGN-U314153	ATTGGTGGAAAGTAACATCATCG	173	1.107 ± 0.0184	[11]
Glyceraldehyde 3-phosphate dehydrogenase	GAPDH	U93208	ACCACAAATTGCCTTGCTCCCTTG	110	1.012 ± 0.0099	[15]
Glyceraldehyde 3-phosphate dehydrogenase	GAPDH	U93208	ATCAACGGTCTTCTGAGTGGCTGT	110	1.012 ± 0.0099	[15]
Ubiquitin 3	UBI	X58253	TCGTAAGGAGTGCCCTAATGCTGA	119	0.956 ± 0.034	[15]
Ubiquitin 3	UBI	X58253	CAATCGCCTCCAGCCTTGTTGTAA	119	0.956 ± 0.034	[15]
Elongation factor 1α	EF1	BT013246	GATTGACAGACGTTCTGGTAAGGA	67	1.040 ± 0.0089	[14]
Elongation factor 1α	EF1	BT013246	ACCGGCATCACCATTCTTCA	67	1.040 ± 0.0089	[14]
Necrotic spotted lesions 1 gene	NECRO	SGN-U581658	AACCTGTTTGCCGGCATCTCCA	193	0.975±0,0062	Our library
Necrotic spotted lesions 1 gene	NECRO	SGN-U581658	GTGTCCCAACATGGCAGAAGGA	193	0.975±0,0062	Our library
Nicotiana lesion-inducing like—HR-like lesion-inducer gene	HR-Li	SGN-U581658	ATTGGCTCCCAAAGTGGCTGGT	154	1.043±0.0117	Our library
	HR-Li	SGN-U581658	AGCACCAGTCATGCTGCCAA	154	1.043±0.0117	Our library

^aAccession numbers from GenBank database or Sol Genomics Network (SGN).

^bAmplification efficiency was calculated using the online real-time PCR Miner tool [25].