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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Lab Invest. 2015 Jun 22;95(9):995–1004. doi: 10.1038/labinvest.2015.71

Figure 4.

Figure 4

FBXW7 promotes the ubiquitination of ENO1. (a) HEK293T cells were co-transfected with Myc-ubiquitin and wild-type FBXW7, FBXW7ΔF or shFBXW7 B and then treated with MG132 for 6 h. Cell lysates were immunoprecipitated with anti-Myc antibody and analyzed by immunoblotting with the anti-ENO1 antibody. Left panel shows the input levels of the indicated proteins. (b) HCT116 FBXW7+/+ and FBXW7−/− cells were transfected with Myc-ubiquitin and treated with MG132 for 6 h. Cell extracts were immunoprecipitated with the anti-Myc antibody and analyzed by immunoblotting with the anti-ENO1 antibody. Left panel shows the input levels of the indicated proteins. All results are representative of three independent experiments.