(A) Histogram of synthetic PRE complex data (gray) modeled with three, non-blurred states. (B) Histogram of synthetic PRE complex data (gray) modeled with two, non-blurred states. The “Avg. State” is a weighted combination of MS I/IS1 and IS2. (C) Histograms and probability distributions of the static EFRET value means generated for the synthetic PRE complex dataset. (D) Plots of the model probability densities minus the normalized histograms from panels A and B. The sum of the squares of these residuals are 10.03, and 4.02 for the three-state and two-state models, respectively, suggesting that the blurred, synthetic, PRE complex data are better represented by a two-state model.