Table 1. Pairwise Changes in phox Protein mRNA Levels when PLB-985 Cells were Maintained for 72 Hours in the Presence or Absence of DMSO and/or IFN-γ.
| Protein | Fold Change | p-value |
|---|---|---|
| mRNA change in IFN-γ treated cells relative to untreated cells | ||
| p22phox | 1.05 | 0.68 |
| gp91phox | 2.48 | 0.000015 |
| p47phox | 1.14 | 0.52 |
| p67phox | 1.68 | 0.0033 |
| p40phox | 1.13 | 0.069 |
| mRNA Change in DMSO treated cells relative to untreated cells | ||
| p22phox | 2.18 | 0.00048 |
| gp91phox | 3.47 | 5.0X10−7 |
| p47phox | 5.3 | 0.0000070 |
| p67phox | 6.89 | 7.3X10−7 |
| p40phox | 2.51 | 0.0000040 |
| mRNA change in DMSO plus IFN-γ treated cells relative to untreated cells | ||
| p22phox | 2.07 | 0.00083 |
| gp91phox | 4.14 | 3.3X10−7 |
| p47phox | 8 | 0.000011 |
| p67phox | 7.33 | 7.2X10−7 |
| p40phox | 2.45 | 0.0000020 |
| mRNA change in DMSO plus IFN-γ treated cells relative to DMSO treated cells | ||
| p22phox | -1.05 | 0.098 |
| gp91phox | 1.19 | 0.010 |
| p47phox | 1.51 | 0.025 |
| p67phox | 1.06 | 0.34 |
| p40phox | -1.02 | 0.86 |
Pairwise comparisons of the changes in phox protein mRNA levels after 72 hours of culture in various conditions. RNA measurement was done using Affymetrix Human Genome 2.0 DNA microarrays. For each phox protein, the inferred fold change in mRNA is shown for the indicated comparisons along with the corresponding statistical significance. The p values were obtained from an ANOVA test. The data is based on 4 independent experiments in which all 4 cell-treatment conditions were carried out.