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. 2015 Aug 28;10(8):e0136615. doi: 10.1371/journal.pone.0136615

Fig 1. Proportions of μ-calpain-positive cells and relative amounts of μ-calpain are elevated among ALL blasts.

Fig 1

A. Representative two-parameter plots (dot plots) resulting from simultaneous staining of BM samples (left panel–ALL, right panel–control) with anti-CD19, anti-CD34 and anti-μ-calpain antibodies. Actual corrected MFI values for calpain signal in CD19+ cells are shown. B. Significant difference between the proportion of μ-calpain-positive cells among ALL blasts and nonmalignant BM B cells. Box-and-whisker plots depict the medians, 25th and 75th percentile and range respectively. Asterisk signifies p = 0.02; N(ALL) = 20, N(control) = 9. C. Amount of μ-calpain is significantly higher in ALL blasts than in nonmalignant B lymphocytes. Comparison of relative intensities (MFI) of μ-calpain–bound antibody in CD19+ ALL blasts (ALL) and non-malignant B cells (control). Asterisk denotes p = 0.03; N(ALL) = 16, N(control) = 9). For the details see Materials and Methods.