Fig 7. Inhibition of calpain activity in vitro induces ALL blast apoptosis in a dose-dependent manner.
Representative cytometric result of the changes in the mitochondrial potential (relative increase of JC1 monomer fluorescence) in ALL blasts incubated over 24 hours without (A) and with calpain inhibitor IV at concentrations of 2 and 4 μM (B, C). An insert in A shows the result of a positive control experiment, where BM blasts were treated with 5 μg/ml chelerythrine. D—The effect of calpain inhibitor IV concentration on apoptosis of ALL blasts. Box-and-whisker plots depict the medians, 25th and 75th percentile and range respectively; N = 6. See Materials and Methods for details.