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. 2015 Aug 28;10(8):e0136692. doi: 10.1371/journal.pone.0136692

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© 2015 Kaur et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) SDS-PAGE analysis of purified full length recombinant AtCyp19-3, and deleted versions AtCyp19-3 _(35–176) and AtCyp19-3 _(71–176). Total proteins were isolated from recombinant E.coli BL21(DE3) before (UI) and after induction (I) with 0.5 mM IPTG, and purified by Ni-NTA affinity column. Arrows indicate the purified recombinant proteins. (B) Confirmation of the purified recombinant proteins (arrows) by immunoblotting with the anti-His antibodies. M: markers.