Table 1. Sequences of primers, annealing temperatures, and enzymes used in PCR-RFLPs and sizes of the fragments obtained.
| SNP | Primer sequences | Fragment size (bp) | Annealing temperature | Restriction enzyme | Fragments in wild-type homozygotes (bp) | Fragments in polymorphic homozygotes (bp) |
|---|---|---|---|---|---|---|
| MMP2 T-1306C | F-5´-CTTCCTAGGCTGGTCCTTACTGA-3´ | 193 | 58°C | Xsp I | 186+7 | 162+24+7 |
| R-5´-CTGAGACCTGAAGAGCTAAAGAGC-3´ | ||||||
| MMP9 C-1562T | F-5´-GCCTGGCACATAGTAGGCCC-3´ | 436 | 60°C | Nla3 | 381+48+7 | 246+135+48+7 |
| R-5´-CTTCCTAGCCAGCCGGCATC-3´ | ||||||
| NOS3 G894T | F-5´-AAGGCAGGAGACAGTGGATGGA-3´ | 248 | 58°C | MboI | 248 | 158+90 |
| R-CCCAGTCAATCCCTTTGGTGCTCA-3´ | ||||||
| NOS3 T–786C | F-5´-CACCCAGGCCCACCCCAACT-3´ | 394 | 58°C | Msp I | 394 | 352+42 |
| R-5´-GCCGCAGGTCGACAGAGAGACT-3´ | ||||||
| NOS3 VNTR | F-5´-CTTACTCTCCACTGCTTTTCAGAG-3´ (Martines et al.) | 394–448 | 58°C | NA | NA | NA |
| R-5´-CGCAGGTCAGCAGAGAGACTAG-3´ |
SNP, single nucleotide polymorphism; F, forward; R, reverse; bp, base pairs; VNTR, variable number of tandem repeats, NA, not applicable