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. 2015 Aug 28;10(8):e0137177. doi: 10.1371/journal.pone.0137177

Fig 4. Involvement of ASK1 in LPS-induced p38MAPK, JNK1/2, C/EBPß and p65 phosphorylation and COX-2 expression in SV-LECs.

Fig 4

Cells were transiently transfected with pcDNA or HA tagged ASK1-DN (HA-ASK1-DN) for 48 h followed by the treatment with LPS (3 mg/ml) for another 20 min. Phosphorylation status of p38MAPK, JNK1/2, C/EBPβ and p65 were then determined by immunoblotting. Figures shown in (A) are representative of at least four independent experiments with similar results. The compiled results of p38MAPK (B), JNK1/2 (C), C/EBPβ (D) and p65 (E) phosphorylations are shown at the bottom of the chart. Each column represents the mean ± S.E.M.. of four independent experiments. *p < 0.05, compared with the pcDNA group treated with LPS alone. F. Cells were transiently transfected with pcDNA or HA-ASK1-DN for 48 h followed by the treatment with LPS (3 mg/ml) for another 24 h. COX-2 level was then determined by immunoblotting. Each column represents the mean ± S.E.M.. of seven independent experiments. *p < 0.05, compared with the pcDNA group treated with LPS alone. G. Cells were treated with LPS (3 μg/ml) for the indicated time periods. Cells were then harvested and ASK1 Thr845 phosphorylation was determined by immunoblotting. Compiled results are shown at the bottom of the chart. Each column represents the mean ± S.E.M. of seven independent experiments. * p<0.05, compared with the control group.