Fig. 6.

Western blot analysis of proteins from two mothers with zinc-deficient infants. Expression levels of SLC30A5 and SLC30A6 protein were analysed in cells from Mother 1 and Mother 2. a For SLC30A5, bands of the predicted size of 57 kDa were detected in all lymphoblasts, and bands of 55 KDa were present in all fibroblasts, for both Mother 1, Mother 2 and controls. A band of 57 kDa was detected in PMC42 cells, and a band of 55 kDa was found in normal breast tissue (BT). b For SLC30A6, two bands of approximately 55 and 57 KDa were detected in lymphoblasts from Mother 1, Mother 2 and control cells, while in fibroblasts a 55 kDa band was found in Mother 2 and controls. A band between 55 and 57 kDa was detected in PMC42 cells and normal breast tissue. An antibody to housekeeping human β-actin, along with Ponceau S, was used to indicate the relative levels of protein, and densitometry was performed to quantify the results. Values represent mean of triplicates ± SEM, *p ≤ 0.05