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Bisulphite pyrosequencing for DNA methylation of SLC30A5 and SLC30A6 genes. Quantitative bisulphite pyrosequencing was used to determine the percentage methylation at individual CpG sites within the SLC30A5 and SLC30A6 promoter regions. a For SLC30A5, lymphoblasts CpG site 2 was significantly less methylated in Mother 1 (4 %) and Mother 2 (4 %) compared with control samples (13 %). b In fibroblasts, Mother 1 methylation (52 %) was greater than in the control sample (38 %)
