. 2015 Aug 29;10(5):38. doi: 10.1007/s12263-015-0487-x

Table 1.

Primers used for RT-PCR and real-time PCR

Forward primer	Reverse primer	PCR product length (bp)
*ZnT1* -RealF1 CTGGTGAACGCCATCTTCCT	*ZnT1* -RealR1 CAATCTCGTGCGGCTCGAT	183
*ZnT2* -RealF2 TGTGATCCTGGTGTTGATGGA	*ZnT2* -RealR2 CAGGCTGTGCAGGGCTTCT	103
*ZnT3* -RealF1 CACCCTCCGAGACGTTCTTC	*ZnT3* -RealR1 GGCACCGACAACAGCGTAT	90
*ZnT4* -RealF2 AACCAGTCTGGTCACCGTCA	*ZnT4* -RealR2 CTATCCTGCCCATGGTTACG	93
*ZnT5* -RealF1 CCAGCGCTCGATTAACAAAATA	*ZnT5* -RealR1 TGTGAACAGCTTTTAGGAGATCA	134
*ZnT6* -RealF2 TCCTTTTTTGGCAAGTTGTTACG	*ZnT6* -RealR2 AAGCAGGAAGCCAGTACATATCAAG	111
*ZnT7* -RealF1 TTGCCCCTGTCCATCAAAG	*ZnT7* -RealF2 AGACCTAAACCAGCCCGAGATC	81
*ZnT8* -RealF1 ACCATGGTCATAGCTTACGA	*ZnT8* -RealF2 CGAATCAGTACCGGCAATTTA	157
*ZnT9* -RealF1 AACGGCATTAGCATCGTATG	*ZnT9* -RealF2 GTAACGTGAAACTTGGATCGAT	114
*ZnT10* -RealF1 AGCTTTCGCAATGGCTTAGG	*ZnT10* -RealF2 GTACCGTAACTGGTTAATATCG	95
*βAC* - *RealF* GACAGGATGCAGAACGAGAT	*βAC* - *RealR* TGATCCACATCTGCTGGAAGGT	138
*GAPDH* - *RealF* CCACCCATGGCAAATTCC	*GAPDH* - *RealR* TGGGATTTCCATTGATGACAA	145
*ZnT5* -A GCAGCGGCGAGACATGAGGAG	*ZnT5* -B CACTACCACTCCTCCAGACAG	1113
*ZnT5* -C TCTCTCATTATGCCTTTTGC	*ZnT5* -D GCATCTTTAATCAGTGGAACA	1125
*ZnT5* -E CAGCATTGGTGTGATCGTATC	*ZnT5* -F AAGATTCCTTGATCCAGTAGT	629
*ZnT6* -A AGAACGGCTTCCGGCGGG	*ZnT6* -B GAGGCATTGGGATTACGTGA	1131
*ZnT6* - C AAGTCTTACTCCAGACAACA	*ZnT6* -D AATGTGAACAAGACTACTAT	758
*ZnT2* - 1F CGTCCTCACTCAGCAACACC	*ZnT2* - 1R TCTTCGTTCCCTCACCTCAC	658
*ZnT2* - 2F AGGACTCCCATTCCCCTATC	*ZnT2* - 2R AGGGAGAATAACGTCACCCATGT	582
*ZnT2* - 3F ATATTGGTGGCCCATTTCAC	*ZnT2* - 3R ACAGAGGCCATGGTTGACAT	503
*ZnT2* - 4F ATATGAGGGGTGGGGTAAGG	*ZnT2* - 4R ACAGCTCCCAGTGTTCTTGG	527
*ZnT2* - 5F GCCCTATCTCTCATGGCTGT	*ZnT2* - 5R CCACCTACCCTTCAGGTTGT	533
*ZnT2* - 6F TTCTGAACTGTGGTCTGTCCTT	*ZnT2* - 6R GAACTGCCAGACCTGAAAGC	501
ZnT2-7F TCAGTGTTAAGAGTGGAGAGGAA	*ZnT2* - 7R AACCCCAGCCTCAGTTTCTT	530
*ZnT2* - 8F TACCTGGCCAGAGGAATGGAA	*ZnT2* - 8R GAAAGGGAACATTTGGCTCA	848
*ZnT2* - 9F TATGAATCTGAGCCCCTCCA	*ZnT2* - 9R CAAGTGACCAAACCCACCTC	912
*ZnT2* - *C5F* ACTGGTGTACCTGGCTGTGG	*ZnT2* - *C5R* CCGAGTAGTCCTCGATCTGG	210
*ZnT5* - *P1F* TCAGGCTCCTCTCATTCTCC	*ZnT5* - *P1R* TGTTTCTTACAGCCCCGAAC	1256
*ZnT5* - *P2F* GGCAAGTCCATAAAGTGAAGC	*ZnT5* - *P2R* CTGAGGAGGAGAATGGCTTG	1189
*ZnT5* - *P3F* CAAGCCATTCTCCTCCTCAG	*ZnT5* - *P3R* GACCAAAATTTGCCTCCTCA	1098
*ZnT5* - *P4F* GAGACAGGGTTTGCCATGT	*ZnT5* - *P4R* CAAGCAGAACACTCGCCTTT	1045
*ZnT5* - *P5F* TGGGAATACAGCCCAGTAGG	*ZnT5* - *P5R* GACCAGATTAATGCCAGAGC	645
*ZnT5* - *P6F* CCATACTGCCCAGACCCTTA	*ZnT5* - *P6R* ATGGCGAAACCCTGTCTCTA	432
*ZnT5* - *P7F* AGCCACAAAAGCAGTCACCT	*ZnT5* - *P7R* AAAATTTGCCTCCTCAGCAA	684
*ZnT5* - *P8F* AGGAGAATCGCTTGAACCTG	*ZnT5* - *P8R* GGAGAAAGTGCATCCTGAGC	750
*ZnT5* - *P9F* TCCCAAATTTCCCAGAGTA	*ZnT5* - *P9R* GTGGGGAGCTTACAAAATGG	243
*ZnT5* - *P10F* CCATACTGCCCAGACCCTTA	*ZnT5* - *P10R* GAGACCAGCCTGACCAACAT	189
*ZnT5* - *P11F* CCATCTGGACCCATCAGTCT	*ZnT5* - *P11R* CCTGGCCCTGAATTCTTTCT	530
*ZnT5* - *C1F* CTGATGACGTGGCTTGGC	*ZnT5* - *C1R* CGAAAAGTCCCACAGCCTTC	120
*ZnT6* - *P1F* AAGGGTGTCAGCAGAGATGG	*ZnT6* - *P1R* GGGACTGTCATGATGGGATT	1170
*ZnT6* - *P2F* CACCTTCTCACCGTGTCCTT	*ZnT6* - *P2R* AGGCAGGAGAATTGCTTGAA	1037
*ZnT6* - *P3F* TCTCACCCAGGATTCATCTC	*ZnT6* - *P3R* TGTAGCACGTGTGTGTTTGTCAGA	1168
*ZnT5* - *P4F* TTAAGCATTTGGGATGAAGGA	*ZnT5* - *P4R* GTGCTCGAGTTTTCCCAGAA	1076
*ZnT5* - *P5F* GGAGGCCAAGGCAGGTAG	*ZnT5* - *P5R* GAATTGCTTGAACCCAGGAG	985
*ZnT5* - *P6F* TTTCTTCCTGGCTTGCAGAT	*ZnT5* - *P6R* GGTTTCACCATGTTGTGCAG	756
*ZnT5* - *P7F* TGGGCAGCTTAAATAACAGAAA	*ZnT5* - *P7R* GTGATTCAGCCTTTGGGAAG	468
*ZnT5* - *P8F* TCAGCAACCCTAATCCAACC	*ZnT5* - *P8R* TGTAGCACGTGTGTTTGTCA	496
*ZnT5* - *P9F* CGAAAAACTCCTCGTTGCTG	*ZnT5* - *P9R* ACTCCAATGACCCCTCCTCT	278
*ZnT5* - *P10F* GGAGGCCAAGGCAGGTAG	*ZnT5* - *P10R* ATGGCAAAACCCCGTCTC	543
*ZnT5* - *P11F* ACCCATCAGAAGAAATAAACCA	*ZnT5* - *P11R* ATTTGAGGCCAGGCACAGT	272
*ZnT6* - *C1F* TTGACTCCTTGGCTTCCAAT	*ZnT6* - *C1R* AAAGGAGAGAAACTTTAACAAAAATC	103
*ZnT2IE* - 1F CGTCCTCACTCAGCAACACC	*ZnT2IE* - 1R TCTTCGTTCCCTCACCTCAC	98
*ZnT2IE* - 2F AGGACTCCCATTCCCCTATC	*ZnT2IE* - 2R AGGAGAATAACGTCACCCATGT	128
*ZnT2IE* - 3F ATATTGGTGGCCCATTTCAC	*ZnT2IE* - 3R ACAGAGGCCATGGTTGACAT	136
*ZnT2IE* - 4F ATATGAGGGGTGGGGTAAGG	*ZnT2IE* - 4R ACAGCTCCCAGTGTTCTTGG	189
*ZnT2IE* - 5F GCCCTATCTCTCATGGCTGT	*ZnT2IE* - 5R CCACCTACCCTTCAGGTTGT	97
*ZnT2IE* - 6F TTCTGAACTGTGGTCTGTCCTT	*ZnT2IE* - 6R GAACTGCCACACCTGAAGC	84
*ZnT2IE* - 7F TCAGTGTTAAGAGTGGAGAGGAA	*ZnT2IE* - 7R AACCCCAGCCTCAGTTTCTT	95
*ZnT2IE* - 8F TACCTGGCAGAGGAATGGAA	*ZnT2IE* - 8R GAAAGGGAACATTTGGCTCA	178
*ZnT2IE* - 9F TATGAATCTGAGCCCCTCCA	*ZnT2IE* - 9R CAAGTGACCAAACCCACCTC	153

Primer names contain the name of the gene, and following abbreviations: Real real-time PCR primer, A–F primers for coding region sequencing, P promoter region primers, IE intron–exon primers

Primers to ZnT4 were designed to GenBank sequence Accession No AF025409

Primers to ZnT1 were designed to GenBank sequence Accession No AF323590

Primers to ZnT2 were designed to GenBank sequence Accession No NM_032513

Primers to ZnT3 were designed to GenBank sequence Accession No U76010

Primers to ZnT5 were designed to GenBank sequence Accession No AF461760

Primers to ZnT6 were designed to GenBank sequence Accession No XM_059432

Primers to ZnT7 were designed to GenBank sequence Accession No AY094606

Primers to ZnT8 were designed to GenBank sequence Accession No AY117411

Primers to ZnT9 were designed to GenBank sequence Accession No BC016949

Primers to ZnT10 were designed to GenBank sequence Accession No AY117411

Primers to β-actin were designed to GenBank sequence Accession No E00829