Table 1.
Forward primer | Reverse primer | PCR product length (bp) |
---|---|---|
ZnT1
-RealF1
CTGGTGAACGCCATCTTCCT |
ZnT1
-RealR1
CAATCTCGTGCGGCTCGAT |
183 |
ZnT2
-RealF2
TGTGATCCTGGTGTTGATGGA |
ZnT2
-RealR2
CAGGCTGTGCAGGGCTTCT |
103 |
ZnT3
-RealF1
CACCCTCCGAGACGTTCTTC |
ZnT3
-RealR1
GGCACCGACAACAGCGTAT |
90 |
ZnT4
-RealF2
AACCAGTCTGGTCACCGTCA |
ZnT4
-RealR2
CTATCCTGCCCATGGTTACG |
93 |
ZnT5
-RealF1
CCAGCGCTCGATTAACAAAATA |
ZnT5
-RealR1
TGTGAACAGCTTTTAGGAGATCA |
134 |
ZnT6
-RealF2
TCCTTTTTTGGCAAGTTGTTACG |
ZnT6
-RealR2
AAGCAGGAAGCCAGTACATATCAAG |
111 |
ZnT7
-RealF1
TTGCCCCTGTCCATCAAAG |
ZnT7
-RealF2
AGACCTAAACCAGCCCGAGATC |
81 |
ZnT8
-RealF1
ACCATGGTCATAGCTTACGA |
ZnT8
-RealF2
CGAATCAGTACCGGCAATTTA |
157 |
ZnT9
-RealF1
AACGGCATTAGCATCGTATG |
ZnT9
-RealF2
GTAACGTGAAACTTGGATCGAT |
114 |
ZnT10
-RealF1
AGCTTTCGCAATGGCTTAGG |
ZnT10
-RealF2
GTACCGTAACTGGTTAATATCG |
95 |
βAC
-
RealF
GACAGGATGCAGAACGAGAT |
βAC
-
RealR
TGATCCACATCTGCTGGAAGGT |
138 |
GAPDH
-
RealF
CCACCCATGGCAAATTCC |
GAPDH
-
RealR
TGGGATTTCCATTGATGACAA |
145 |
ZnT5
-A
GCAGCGGCGAGACATGAGGAG |
ZnT5
-B
CACTACCACTCCTCCAGACAG |
1113 |
ZnT5
-C
TCTCTCATTATGCCTTTTGC |
ZnT5
-D
GCATCTTTAATCAGTGGAACA |
1125 |
ZnT5
-E
CAGCATTGGTGTGATCGTATC |
ZnT5
-F
AAGATTCCTTGATCCAGTAGT |
629 |
ZnT6
-A
AGAACGGCTTCCGGCGGG |
ZnT6
-B
GAGGCATTGGGATTACGTGA |
1131 |
ZnT6
-
C
AAGTCTTACTCCAGACAACA |
ZnT6
-D
AATGTGAACAAGACTACTAT |
758 |
ZnT2
-
1F
CGTCCTCACTCAGCAACACC |
ZnT2
-
1R
TCTTCGTTCCCTCACCTCAC |
658 |
ZnT2
-
2F
AGGACTCCCATTCCCCTATC |
ZnT2
-
2R
AGGGAGAATAACGTCACCCATGT |
582 |
ZnT2
-
3F
ATATTGGTGGCCCATTTCAC |
ZnT2
-
3R
ACAGAGGCCATGGTTGACAT |
503 |
ZnT2
-
4F
ATATGAGGGGTGGGGTAAGG |
ZnT2
-
4R
ACAGCTCCCAGTGTTCTTGG |
527 |
ZnT2
-
5F
GCCCTATCTCTCATGGCTGT |
ZnT2
-
5R
CCACCTACCCTTCAGGTTGT |
533 |
ZnT2
-
6F
TTCTGAACTGTGGTCTGTCCTT |
ZnT2
-
6R
GAACTGCCAGACCTGAAAGC |
501 |
ZnT2-7F
TCAGTGTTAAGAGTGGAGAGGAA |
ZnT2
-
7R
AACCCCAGCCTCAGTTTCTT |
530 |
ZnT2
-
8F
TACCTGGCCAGAGGAATGGAA |
ZnT2
-
8R
GAAAGGGAACATTTGGCTCA |
848 |
ZnT2
-
9F
TATGAATCTGAGCCCCTCCA |
ZnT2
-
9R
CAAGTGACCAAACCCACCTC |
912 |
ZnT2
-
C5F
ACTGGTGTACCTGGCTGTGG |
ZnT2
-
C5R
CCGAGTAGTCCTCGATCTGG |
210 |
ZnT5
-
P1F
TCAGGCTCCTCTCATTCTCC |
ZnT5
-
P1R
TGTTTCTTACAGCCCCGAAC |
1256 |
ZnT5
-
P2F
GGCAAGTCCATAAAGTGAAGC |
ZnT5
-
P2R
CTGAGGAGGAGAATGGCTTG |
1189 |
ZnT5
-
P3F
CAAGCCATTCTCCTCCTCAG |
ZnT5
-
P3R
GACCAAAATTTGCCTCCTCA |
1098 |
ZnT5
-
P4F
GAGACAGGGTTTGCCATGT |
ZnT5
-
P4R
CAAGCAGAACACTCGCCTTT |
1045 |
ZnT5
-
P5F
TGGGAATACAGCCCAGTAGG |
ZnT5
-
P5R
GACCAGATTAATGCCAGAGC |
645 |
ZnT5
-
P6F
CCATACTGCCCAGACCCTTA |
ZnT5
-
P6R
ATGGCGAAACCCTGTCTCTA |
432 |
ZnT5
-
P7F
AGCCACAAAAGCAGTCACCT |
ZnT5
-
P7R
AAAATTTGCCTCCTCAGCAA |
684 |
ZnT5
-
P8F
AGGAGAATCGCTTGAACCTG |
ZnT5
-
P8R
GGAGAAAGTGCATCCTGAGC |
750 |
ZnT5
-
P9F
TCCCAAATTTCCCAGAGTA |
ZnT5
-
P9R
GTGGGGAGCTTACAAAATGG |
243 |
ZnT5
-
P10F
CCATACTGCCCAGACCCTTA |
ZnT5
-
P10R
GAGACCAGCCTGACCAACAT |
189 |
ZnT5
-
P11F
CCATCTGGACCCATCAGTCT |
ZnT5
-
P11R
CCTGGCCCTGAATTCTTTCT |
530 |
ZnT5
-
C1F
CTGATGACGTGGCTTGGC |
ZnT5
-
C1R
CGAAAAGTCCCACAGCCTTC |
120 |
ZnT6
-
P1F
AAGGGTGTCAGCAGAGATGG |
ZnT6
-
P1R
GGGACTGTCATGATGGGATT |
1170 |
ZnT6
-
P2F
CACCTTCTCACCGTGTCCTT |
ZnT6
-
P2R
AGGCAGGAGAATTGCTTGAA |
1037 |
ZnT6
-
P3F
TCTCACCCAGGATTCATCTC |
ZnT6
-
P3R
TGTAGCACGTGTGTGTTTGTCAGA |
1168 |
ZnT5
-
P4F
TTAAGCATTTGGGATGAAGGA |
ZnT5
-
P4R
GTGCTCGAGTTTTCCCAGAA |
1076 |
ZnT5
-
P5F
GGAGGCCAAGGCAGGTAG |
ZnT5
-
P5R
GAATTGCTTGAACCCAGGAG |
985 |
ZnT5
-
P6F
TTTCTTCCTGGCTTGCAGAT |
ZnT5
-
P6R
GGTTTCACCATGTTGTGCAG |
756 |
ZnT5
-
P7F
TGGGCAGCTTAAATAACAGAAA |
ZnT5
-
P7R
GTGATTCAGCCTTTGGGAAG |
468 |
ZnT5
-
P8F
TCAGCAACCCTAATCCAACC |
ZnT5
-
P8R
TGTAGCACGTGTGTTTGTCA |
496 |
ZnT5
-
P9F
CGAAAAACTCCTCGTTGCTG |
ZnT5
-
P9R
ACTCCAATGACCCCTCCTCT |
278 |
ZnT5
-
P10F
GGAGGCCAAGGCAGGTAG |
ZnT5
-
P10R
ATGGCAAAACCCCGTCTC |
543 |
ZnT5
-
P11F
ACCCATCAGAAGAAATAAACCA |
ZnT5
-
P11R
ATTTGAGGCCAGGCACAGT |
272 |
ZnT6
-
C1F
TTGACTCCTTGGCTTCCAAT |
ZnT6
-
C1R
AAAGGAGAGAAACTTTAACAAAAATC |
103 |
ZnT2IE
-
1F
CGTCCTCACTCAGCAACACC |
ZnT2IE
-
1R
TCTTCGTTCCCTCACCTCAC |
98 |
ZnT2IE
-
2F
AGGACTCCCATTCCCCTATC |
ZnT2IE
-
2R
AGGAGAATAACGTCACCCATGT |
128 |
ZnT2IE
-
3F
ATATTGGTGGCCCATTTCAC |
ZnT2IE
-
3R
ACAGAGGCCATGGTTGACAT |
136 |
ZnT2IE
-
4F
ATATGAGGGGTGGGGTAAGG |
ZnT2IE
-
4R
ACAGCTCCCAGTGTTCTTGG |
189 |
ZnT2IE
-
5F
GCCCTATCTCTCATGGCTGT |
ZnT2IE
-
5R
CCACCTACCCTTCAGGTTGT |
97 |
ZnT2IE
-
6F
TTCTGAACTGTGGTCTGTCCTT |
ZnT2IE
-
6R
GAACTGCCACACCTGAAGC |
84 |
ZnT2IE
-
7F
TCAGTGTTAAGAGTGGAGAGGAA |
ZnT2IE
-
7R
AACCCCAGCCTCAGTTTCTT |
95 |
ZnT2IE
-
8F
TACCTGGCAGAGGAATGGAA |
ZnT2IE
-
8R
GAAAGGGAACATTTGGCTCA |
178 |
ZnT2IE
-
9F
TATGAATCTGAGCCCCTCCA |
ZnT2IE
-
9R
CAAGTGACCAAACCCACCTC |
153 |
Primer names contain the name of the gene, and following abbreviations: Real real-time PCR primer, A–F primers for coding region sequencing, P promoter region primers, IE intron–exon primers
Primers to ZnT4 were designed to GenBank sequence Accession No AF025409
Primers to ZnT1 were designed to GenBank sequence Accession No AF323590
Primers to ZnT2 were designed to GenBank sequence Accession No NM_032513
Primers to ZnT3 were designed to GenBank sequence Accession No U76010
Primers to ZnT5 were designed to GenBank sequence Accession No AF461760
Primers to ZnT6 were designed to GenBank sequence Accession No XM_059432
Primers to ZnT7 were designed to GenBank sequence Accession No AY094606
Primers to ZnT8 were designed to GenBank sequence Accession No AY117411
Primers to ZnT9 were designed to GenBank sequence Accession No BC016949
Primers to ZnT10 were designed to GenBank sequence Accession No AY117411
Primers to β-actin were designed to GenBank sequence Accession No E00829