Fig 3. Photoactivatable reassembly of split Venus fluorescence and split RhoA.
(A) General schematic diagram of reassembly of split Venus fluorescence after blue-light photostimulation. (B) HeLa cells co-expressing VenusN-InN and LOVInC-VenusC showing the restoration of YFP fluorescence after photostimulation with blue-light. (C) HeLa co-expressing split Venus fused to its respective intein fragment as control. (D) General schematic diagram of reassembly of split RhoA by photoactivatable PTS activity. Expression of (E) RhoAN-InN-mRFP or (F) LOVInC-RhoAC-Venus alone did not result in continuous dynamic blebbing. (G-L) However, co-expression of the two constructs resulted in continuous dynamic blebbing in HeLa cells stemming from the functional reassembled RhoA after photostimulated PTS activity (n = 10 experiments). Scale bars are 10 μm. Images are in false colour.