Table 1.
Experimental condition for bioassay using cVA
| References | Bioassay | CVA applied | Application | Chamber size | Odor context |
|---|---|---|---|---|---|
| Jallon et al. (1981) | Courtship inhibition | 220 ng | |||
| Mane et al. (1983) | Copulation inhibition | 200 ng | 0.1 µl acetone | 0.5 ml (0.5 cm3) | |
| Bartelt et al. (1985) | Aggregation | 150 ng–150 µg | 80 cm × 40 cm × 40 cm (128 × 103 cm3) | Instant medium + yeast | |
| Zawistowski and Richmond (1986) | Courtship inhibition | 200 ng | 0.1 µl acetone | 0.2 cc (0.2 cm3) | |
| copulation inhibition | 100 ng | 0.1 µl acetone | |||
| Wertheim et al. (2002) | Aggregation | 4.5 µg | 15 µl hexane | 30 cm × 40 cm × 60 cm (72 × 103 cm3) | Mashed apple, yeast |
| Xu et al. (2005) | Aggregation | 1 % | Unknown | 10 cm × 2 cm petri dish (157 cm3) | |
| Ejima et al. (2007) | Courtship memory | 0.2 ng–200 µg | Hexane | 8 mm diameter × 6 mm height (0.3 cm3) | |
| Courtship inhibition | 200 µg | Hexane | |||
| Kurtovic et al. (2007) | Courtship inhibition | 10 % (≈18 ng) | 0.2 µl acetone | 10 mm diameter × 4 mm height (0.3 cm3) | |
| Schlief and Wilson (2007) | Aggregation | 0.4 % (≈900 ng) | 250 µl water | Y-maze using two 2L flasks (2 × 103 cm3) | Propionic acid |
| Griffith and Ejima (2009) | Courtship inhibition | 200 ng | hexane | 8 mm diameter × 6 mm height (0.3 cm3) | Yeast paste |
| Wang and Anderson (2010) | Aggression | 100–500 µg | Acetone | 50 cm × 40 cm × 120 cm height (240 × 103 cm3) | Apple juice + sugar |
| Copulation inhibition | 5 mg | Acetone | |||
| Billeter et al. (2009) | Copulation inhibition | 1 µg | Hexane | 10 mm diameter × 5 mm height (0.4 cm3) | |
| Ronderos and Smith (2010) | Courtship inhibition | 1 µl (≈900 ng) | (Pure) | 1.5 cm diameter | |
| Chertemps et al. (2012) | Courtship inhibition | 150 ng | Acetone | 3 cm diameter × 0.5 cm height (3.5 cm3) | |
| Thistle et al. (2012) | Courtship inhibition | 200 ng | Ethanol | Falcon 48-well plate (1.35 cm3) | Grape juice |