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. 2015 Aug 28;10(8):e0136394. doi: 10.1371/journal.pone.0136394

Fig 6. Effects of ionizing radiation in IgE-mediated passive cutaneous anaphylaxis and the late-phase cutaneous model.

Fig 6

Anti-DNP (20 ng) was injected intradermally in the left ear, whereas the right ear was injected with saline as a control. After 24 h, mice were irradiated with 0–0.5 Gy before injection of 100 μg DNP-HAS containing Evan’s blue dye via the tail vein. (A) After 30 min, ear punches (8 mm) from both ears were collected and used for extraction of the Evan’s blue dye (B). The optical density was measured at 620 nm. (C) Mice were passively sensitized by i.v. injection of 2 μg anti-DNP IgE. After 24 h, mice were irradiated with 0–0.5 Gy before 20 μl of 0.3% DNFB in acetone/olive oil (4:1) was applied epicutaneously to both sides of the left hind paw and left ear. Acetone/olive oil (20 μl) was applied to the right ear and right hind paw as a control. After 24 h, the thicknesses of the ear and hind paw were measured using a digital microcaliper. Data represent the mean ± SEM from five independent experiments. *p<0.05, #p < 0.05 versus the corresponding control group of foot and ear, respectively.