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. 2015 Aug 28;10(8):e0134087. doi: 10.1371/journal.pone.0134087

Fig 7. Unfolded protein response and proinflammatory gene expression profiling in primary astroyctes.

Fig 7

Primary astrocytes were treated with 0.1 or 0.5 mM Palmitate (Pal) for 48 hours. mRNA and protein were isolated for RT-PCR, western blotting and ELISA analysis. UPR (ATF4 (A) and Bip (B)) and proinflammatory (IL1beta (C) and TNFα(D)) gene expression were measured by qRT-PCR in primary astrocytes. The protein level of ATF4 (E), Bip (F) and IL1beta (G) were determined by western blotting analysis. Quantification of western blot signals was normalized by actin expression and compared to control. H. ELISA analysis of TNFα secretion in the media of astrocyte culture with palmitate treatment. The quantification was compared to control (n = 4). Results were compared to control and presented as mean±SEM, *p<0.05, **<0.01, ANOVA with Student-Newman-Keuls post hoc analysis.