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Oligomerization of wt and V71M-mutated GlpF at increasing IPTG concentrations. (A) The oligomerization tendency of the wt (
) and V71M-mutated GlpF (
) was analyzed in vivo at increasing IPTG concentrations using the GALLEX assay (n = 6 ± SE). Plotted is the rel. β-gal. act. vs. the IPTG concentration. (B) Western blot analyses, probing the expression of GlpF-wt and GlpF-V71M in E. coli membranes, were performed using an antibody directed against the GlpF C-terminus.
