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Activity of GlpF-wt, GlpF-E43A and GlpF-V71M. (A) Typical light scattering curves are shown, observed using E. coli SK46 cells expressing GlpF-wt (green), GlpF-E43A (gray), GlpF-V71M (beige) or E. coli cells transformed with an empty vector (black). (B) Western blot analyses, probing the expression of GlpF-wt, GlpF-E43A and GlpF-V71M in E. coli SK46 membranes at an IPTG concentration of 500 μM, were performed using an antibody directed against the GlpF C-terminus.
