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. 2015 Aug 28;10(8):e0136362. doi: 10.1371/journal.pone.0136362

Fig 6. The ID domains of Sgt2p and Fus mediate their localization to Htt-polyQ aggregates in yeast cells.

Fig 6

(A, B) Western blots of lysates from yeast strain W303 expressing HttQ25-GFP or HttQ103-GFP in combination with HA-tagged Sgt2p or Sgt2ΔID (A = αGFP; B = αHA). (C, D) Western blots of cells expressing HttQ25-GFP or HttQ103-GFP in combination with FUS or FUSΔID (C = αGFP; D = FUS & α-β actin). Because FUS is quickly degraded in non-denaturing conditions, input controls using urea lysis of cells were included to show initial protein loads. (E, F) Western blots of FUS or FUS(4FL) in HttQ25-GFP-expressing or HttQ103-GFP-expressing cells.