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. 2015 Aug 28;10(8):e0136938. doi: 10.1371/journal.pone.0136938

Fig 9. CDR region mutations do not impair Rab3 function to control Brp distribution.

Fig 9

(A) Images of NMJs costained with α-Brp (red) and α-DGluRIII (green) from WT (dvglut NMJX-Gal4/+), the rab3 mutant (dvglut NMJX-Gal4/+; rab3 rup/Df(2R)ED2076), the rab3 mutant expressing the UAS-rab3FDY18-20AAA transgene (dvglut NMJX-Gal4/+; rab3 rup/Df(2R)ED2076; UAS-rab3FDY18-20AAA/+), the rab3 mutant expressing the UAS-rab3WDN124-126AAA transgene (dvglut NMJX-Gal4/+; rab3 rup/Df(2R)ED2076; UAS-rab3WDN124-126AAA/+), the rab3 mutant expressing the UAS-rab3KM185-186AA transgene (dvglut NMJX-Gal4/+; rab3 rup/Df(2R)ED2076; UAS-rab3KM185-186AA/+), and the rab3 mutant expressing the UAS-rab3SL189-190AA transgene (dvglut NMJX-Gal4/+; rab3 rup/Df(2R)ED2076; UAS-rab3SL189-190AA/+). Scale bar, 2μm. (B-C) Histograms show (B) the average percentage of DGluRIII clusters apposed to Brp puncta per NMJ and (C) the average area of individual Brp puncta for the genotypes listed in (A). n = 8 NMJs for all genotypes; *p<<0.001.