Fig 5. cfDNA and liver metastasis DNA are well correlated.

A) cfDNA variant allele percentage is correlated with liver metastasis variant allele percentage B) Maximum parsimony tree showing relatedness of samples, branch length are number of somatic, nonsynonymous mutations C) Seventeen additional mutations were identified uniquely in cfDNA, 9 of which have reads supporting them in the primary and/or met, but where not called due to insufficient sequencing depth or variant allele percentage. Genes in red were successfully validated on the Ion Torrent PGM, genes in blue failed to validate, genes were black were not validated.