Table 1. Lymphocytes composition of spleen, lymph nodes, and thymus in PEA-15 -/—mice.
The cellular composition of spleen, lymph nodes, and thymus for PEA-15 -/- mice and their WT littermates was determined by cell counting and/or flow cytometry after staining with mAbs specific for lymphocyte subsets. (N = 6; **: P< 0.05).
| Spleen | ||
| PEA-15 +/+ | PEA-15 -/- | |
| Number of total splenocytes x 106 | ||
| B cells | 40,4 ± 18.2 | 24.8 ± 5.1 |
| CD4+8- T cells | 19.9 ± 1.8 | 16 ± 5.1 ** |
| CD4-8+ T cells | 12.6 ± 2.9 | 8.9 ± 1.5 ** |
| Lymph nodes | ||
| PEA-15 +/+ | PEA-15 -/- | |
| % among total leukocytes | ||
| B cells | 16.4 ± 3.5 | 14.6 ± 4.5 |
| CD4+8- T cells | 43.5 ± 11 | 40.8 ± 7.9 |
| CD4-8+ T cells | 23.4 ± 3.5 | 22.9 ± 6 |
| % among CD4+ T cells | ||
| CD62LhighCD44low | 75.8 ± 4.2 | 75.3 ± 7.4 |
| CD62LhighCD44high | 5.7 ± 2.5 | 3.2 ± 1.1 ** |
| CD62LlowCD44high | 10.4 ± 1.9 | 9.3 ± 3.5 |
| % among CD8+ T cells | ||
| CD62LhighCD44low | 88.0 ± 2.1 | 87.8 ± 2.1 |
| CD62LhighCD44high | 5.9 ± 1.6 | 4.6 ± 1.3 |
| CD62LlowCD44high | 2.4 ± 0.7 | 2.9 ± 0.8 |
| Thymus | ||
| PEA-15 +/+ | PEA-15 -/- | |
| % among total thymocytes | ||
| CD4+ 8- | 9.4 ± 0.5 | 11.7 ± 1.2 |
| CD4+ 8+ | 76.3 ± 2.3 | 68.1 ± 2.4 |
| CD4- 8- | 11.4 ± 3.1 | 16.5 ± 3 |
| CD4- 8+ | 2.9 ± 0.6 | 3.6 ± 0.6 |