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. 2015 Sep 1;4:e08997. doi: 10.7554/eLife.08997
Reagent Type Manufacturer Catalog # Comments
MV4;11 Human cell line ATCC CRL-9591
K-562 Human cell line ATCC CCL-243
I-BET151 (GSK1210151A) Small molecule Sigma–Aldrich SML0666
DMSO, molecular biology grade Reagent Sigma–Aldrich D8418 Original brand not specified
RPMI-1640 medium, with L-glutamine and sodium bicarbonate Cell culture reagent Sigma–Aldrich R8758 Original catalog number not specified
Fetal bovine serum (FBS) Cell culture reagent Sigma–Aldrich F2442 Original brand not specified
Penicillin–streptomycin solution (100×) stabilized Cell culture reagent Sigma–Aldrich P4333 Original brand not specified
48-well tissue culture plates Labware Corning 3548 Original brand not specified
RNAspin mini RNA isolation Sigma–Aldrich GE25-0500-70 Replaces Qiagen cat. no. 74104 used in original study
Nuclease-free water (DEPC-treated) Chemical Sigma–Aldrich 95284 Reagent needed for RNAspin Mini protocol
96-well plates (for quantification of RNA) Labware Corning 3635 UV/Vis 96-well clear plates for use on Molecular Devices Spectramax 190
First-strand cDNA synthesis kit cDNA synthesis Sigma–Aldrich GE27-9261-01 Replaces Invitrogen cat. no. 28025-013 used in original study
BCL2-primers (forward and reverse) Nucleic acid Sequences listed below in procedure; specific brand information will be left up to the discretion of the replicating lab and recorded later
B2M-primers (forward and reverse) Nucleic acid
96-well multiplate PCR plates, clear qPCR Bio-Rad MLL9601 Original brand not specified
qPCR plate seals Labware Bio-Rad MSB1001 Or equivalent optically clear seals will be used
SYBR® green PCR master mix qPCR Life Technologies 4344463
DNA engine opticon system (qRT-PCR) Instrument Bio-Rad n/a Replaces ABI 7900
Opticon monitor Software Bio-Rad n/a