MV4;11 |
Human cell line |
ATCC |
CRL-9591 |
– |
K-562 |
Human cell line |
ATCC |
CCL-243 |
– |
I-BET151 (GSK1210151A) |
Small molecule |
Sigma–Aldrich |
SML0666 |
– |
DMSO, molecular biology grade |
Reagent |
Sigma–Aldrich |
D8418 |
Original brand not specified |
RPMI-1640 medium, with L-glutamine and sodium bicarbonate |
Cell culture reagent |
Sigma–Aldrich |
R8758 |
Original catalog number not specified |
Fetal bovine serum (FBS) |
Cell culture reagent |
Sigma–Aldrich |
F2442 |
Original brand not specified |
Penicillin–streptomycin solution (100×) stabilized |
Cell culture reagent |
Sigma–Aldrich |
P4333 |
Original brand not specified |
48-well tissue culture plates |
Labware |
Corning |
3548 |
Original brand not specified |
RNAspin mini |
RNA isolation |
Sigma–Aldrich |
GE25-0500-70 |
Replaces Qiagen cat. no. 74104 used in original study |
Nuclease-free water (DEPC-treated) |
Chemical |
Sigma–Aldrich |
95284 |
Reagent needed for RNAspin Mini protocol |
96-well plates (for quantification of RNA) |
Labware |
Corning |
3635 |
UV/Vis 96-well clear plates for use on Molecular Devices Spectramax 190 |
First-strand cDNA synthesis kit |
cDNA synthesis |
Sigma–Aldrich |
GE27-9261-01 |
Replaces Invitrogen cat. no. 28025-013 used in original study |
BCL2-primers (forward and reverse) |
Nucleic acid |
Sequences listed below in procedure; specific brand information will be left up to the discretion of the replicating lab and recorded later |
B2M-primers (forward and reverse) |
Nucleic acid |
96-well multiplate PCR plates, clear |
qPCR |
Bio-Rad |
MLL9601 |
Original brand not specified |
qPCR plate seals |
Labware |
Bio-Rad |
MSB1001 |
Or equivalent optically clear seals will be used |
SYBR® green PCR master mix |
qPCR |
Life Technologies |
4344463 |
– |
DNA engine opticon system (qRT-PCR) |
Instrument |
Bio-Rad |
n/a |
Replaces ABI 7900 |
Opticon monitor |
Software |
Bio-Rad |
n/a |
– |