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. 2015 Sep 1;4:e08997. doi: 10.7554/eLife.08997
Reagent Type Manufacturer Catalog # Comments
MV4;11 Human cell line ATCC CRL-9591
I-BET151 (GSK1210151A) Small molecule Sigma–Aldrich SML0666
DMSO, molecular biology grade Reagent Sigma–Aldrich D1435 Original brand not specified
RPMI-1640 medium, with L-glutamine and sodium bicarbonate Cell culture reagent Gibco, Life Technologies 22400-089 Original catalog number not specified
Fetal bovine serum (FBS) Cell culture reagent Sigma–Aldrich F2442 Original brand not specified
Penicillin–streptomycin solution (100×) stabilized Cell culture reagent Invitrogen 15140122 Original brand not specified
Phosphate buffered saline (PBS) Buffer Gibco, Life Technologies 14190-136
Female and male NOD-SCID mice (6–8 weeks old) Animal model Jackson Laboratory 001303
IV Busulfex (busulfan) Chemical Otsuka America Pharmaceutical, Inc. NDC 59148-070-90 Not originally used
Ammonium chloride solution Chemical Stem Cell Technologies 07850 Replaces red blood cell lysis buffer from 5 prime
CountBright absolute counting beads Flow cytometry reagent Life Technology C36950 Not originally used
FACS lysing solution Chemical Becton–Dickinson 349202 Replaces red blood cell lysis buffer from 5 prime
½ cc LO-DOSE U-100 insulin syringe 28G Labware Becton–Dickinson 329461 Original brand not specified
APC anti-human HLA-A,B,C antibody Antibodies Biolegend 311410 Original catalog number not specified
APC mouse IgG2a, κ isotype control antibody Antibodies Biolegend 400220
Annexin V-FITC Kit Antibodies Miltenyi Biotec Ltd 130-092-052 Original catalog number not specified
7-AAD Dye BD Pharmingen 51-68981E Original catalog number not specified
Kleptose HPB Chemical Roquette Pharma n/a Original brand not specified
0.9% NaCl, USP Chemical Hospira, Inc 0490-1966-05 Original brand not specified
1cc insulin syringe U-100 27G 5/8 Labware Becton–Dickinson 329412 Original brand not specified
Flow cytometer Instrument Becton–Dickinson n/a Canto or LSR II (replaces CyAn ADP from Dako)
FlowJo software Software Tree Star, Inc n/a