MV4;11 |
Human cell line |
ATCC |
CRL-9591 |
– |
I-BET151 (GSK1210151A) |
Small molecule |
Sigma–Aldrich |
SML0666 |
– |
DMSO, molecular biology grade |
Reagent |
Sigma–Aldrich |
D1435 |
Original brand not specified |
RPMI-1640 medium, with L-glutamine and sodium bicarbonate |
Cell culture reagent |
Gibco, Life Technologies |
22400-089 |
Original catalog number not specified |
Fetal bovine serum (FBS) |
Cell culture reagent |
Sigma–Aldrich |
F2442 |
Original brand not specified |
Penicillin–streptomycin solution (100×) stabilized |
Cell culture reagent |
Invitrogen |
15140122 |
Original brand not specified |
Phosphate buffered saline (PBS) |
Buffer |
Gibco, Life Technologies |
14190-136 |
– |
Female and male NOD-SCID mice (6–8 weeks old) |
Animal model |
Jackson Laboratory |
001303 |
– |
IV Busulfex (busulfan) |
Chemical |
Otsuka America Pharmaceutical, Inc. |
NDC 59148-070-90 |
Not originally used |
Ammonium chloride solution |
Chemical |
Stem Cell Technologies |
07850 |
Replaces red blood cell lysis buffer from 5 prime |
CountBright absolute counting beads |
Flow cytometry reagent |
Life Technology |
C36950 |
Not originally used |
FACS lysing solution |
Chemical |
Becton–Dickinson |
349202 |
Replaces red blood cell lysis buffer from 5 prime |
½ cc LO-DOSE U-100 insulin syringe 28G |
Labware |
Becton–Dickinson |
329461 |
Original brand not specified |
APC anti-human HLA-A,B,C antibody |
Antibodies |
Biolegend |
311410 |
Original catalog number not specified |
APC mouse IgG2a, κ isotype control antibody |
Antibodies |
Biolegend |
400220 |
– |
Annexin V-FITC Kit |
Antibodies |
Miltenyi Biotec Ltd |
130-092-052 |
Original catalog number not specified |
7-AAD |
Dye |
BD Pharmingen |
51-68981E |
Original catalog number not specified |
Kleptose HPB |
Chemical |
Roquette Pharma |
n/a |
Original brand not specified |
0.9% NaCl, USP |
Chemical |
Hospira, Inc |
0490-1966-05 |
Original brand not specified |
1cc insulin syringe U-100 27G 5/8 |
Labware |
Becton–Dickinson |
329412 |
Original brand not specified |
Flow cytometer |
Instrument |
Becton–Dickinson |
n/a |
Canto or LSR II (replaces CyAn ADP from Dako) |
FlowJo software |
Software |
Tree Star, Inc |
n/a |
– |